Laser Scanning Confocal Microscope Maximum Magnification

The confocal laser scanning microscope s aim was not to further increase magnification but to make clearer.

Laser scanning confocal microscope maximum magnification.

A confocal laser scanning microscope or also known as laser scanning confocal microscope is used to obtain high resolution images and generate 3d reconstructions through direct optical sectioning to provide clear images from a range of depths of thicker specimens for nanometer level imaging and measurement. The laser scanning microscope uses a scanning design called beam scanning where the laser image path is scanned in a raster pattern on the surface of the sample. In the past the traditional laser microscope excited the whole thickness of the sample resulting in saturated blurry images and sometimes visualizing false colocalization images. The confocal laser scanning microscope clsm is a microscope which focuses only on a single focal plane and the unfocused plane will not be visualized.

The scanning electron microscope has a magnification range from 15x to 200 000x reached in 25 steps and a resolution of 5. Laser scanning confocal microscopy laser scanning confocal microscopes employ a pair of pinhole apertures to limit the specimen focal plane to a confined volume approximately a micron in size. Capturing multiple two dimensional images at different depths in a sample enables the. This resulted in a costly microscope system with magnification limited to 1000x.

Relatively thick specimens can be imaged in successive volumes by acquiring a series of sections along the optical z axis of the microscope. The laser scans across the object and an image is built up pixel by pixel on a screen.