Laser capture microdissection lcm is a method to procure subpopulations of tissue cells under direct microscopic visualization. When applied to plant cells and especially to woody tissues lcm requires extensive optimization to overcome such factors as rigid cell walls large central vacuoles intercellular spaces and technical issues with thickness and flatness of the sections. Laser capture microdissection lcm is a new technique that provides rapid and reliable procurement of specific cells for molecular and other types of analyses.
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